In-Vitro Study I on Alpha PSP 02
Title: In-Vitro Alzheimer Model Study with Neuroblastoma Cells (Sawatsri et al.)
Conclusion and Discussion
In-Vitro studies of neuroblastoma (neuron cells) showed 100% cell death and sever damage of dendrites when neurotoxins were induced. However, when the neuroblastoma (neuron cells) were pretreated with Alpha PSP 02, In-Vitro studies showed different levels of cell survival andapparent recovery of dendrites from teh neurotoxin injuries within 48 hours.

In-Vitro Study II on Alpha PSP 02
Title: Neuroprotective Effective of Alpha PSP 02 in Cell Line Models of Alzheimer's Disease (Sawatsri et al.)
Neuroprotective effect of 0.033, 0.066 and 0.33 mg/ml of Alpha PSP 02 (1:1000, 1:500, 1:100) on LA-N5 that was induced by a 20 min. exposure to 30 μΜ H2O2 (Hydrogen peroxide-induced toxicity). The highest percentage of cells viability was 54.5% as estimated by colorimetric MTT (tetrazolium) Assay. The value represent mean ±SEM of at least three separate experiments, each performed in triplicate, p < 0.05 compared with control group.

The percentage of cells viability of neuroblastoma cell at 0.33 mg/ml of Alpha PSP 02 increase 54%. A Study's t-test (two-tailed) was used to determine p-value of the experiment and showed that p-value was less than 0.05 (p < 0.05). It was found that the percentage of cells viability at 0.33 mg/ml Alpha 02 (experimental group) and a control group were significantly different. From the result it was shown that 0.33 mg/ml Alpha PSP 02 can significantly survive from hydrogen peroxide-induced toxicity when compared with control group.

Alpha PSP 02 showed dose dependent for neuroprotective effect in AD model and 1:100 Alpha PSP 02 demonstated the optimal effect with a significant increase of ATP in mitochondria metabolism of about 54% when compared with control. Alpha PSP 02 has a role to intervention in AD.

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